Essays physics

Bravery Essays and essays, Research Papers. different weather conditions and with a small supply of food and a survival kit. I admire the bravery of the character Pi towards . everything and his positive attitude- He never gave up even though he was forced to live in a boat with a tiger. The principle of “survival” is applicable. Pi shows us that we must not give up when we do anything, no matter how hard it is. The theme of about in spanish bravery and physics, courage is very relevant to our world today because, we usually find ourselves not wanting to environment, do. Courage , Figure 8 , Life of Pi 1966 Words | 5 Pages. Physics. idea that was worth learning about was bravery . Bravery comes in many different forms that people don’t always recognize it as . being bravery . In this novel Mrs. Dubose, Atticus and Jean Louise (Scout), each display an act of bravery that are all different in their own way. Bravery was worth learning about in this novel because we learn how to interpret and see different types of it, not just the big acts that everyone else see’s. Mrs.

Dubose’s act of bravery was very personal and important to her. Prompts. Atticus Finch , Harper Lee , Monroeville, Alabama 1123 Words | 3 Pages. be the feature of bravery ; bravely moving into new unknown work, or sticking with ill proven concepts. In the novel The Alchemist by Paulo . Coelho the physics author puts forward heavy usage of bravery in environment essay the concept of his book. Within the text the protagonist Santiago has several experiences in physics which he displays bravery through his ability to remain focused and take risks. Essay Newspaper. In order for one to successfully accomplish his/her personal legend, one must put forward multiple acts of essays bravery . Throughout Santiago’s. Courage , Forward , Hero 1027 Words | 3 Pages. Voices of War Essay James Blunt’s song “No Bravery ” and the song “I was only 19” by nyu admission essay, Red Gum express similar ideas about war and use many of . the same techniques to present these ideas. Both strongly use imagery and personification to convey a sense of the essays horrors of war and essay, the lasting effect that it has both physically and psychologically on those who are involved both as soldiers and also the innocent victims who are damaged by wars that occur around them. Blunt’s poem was written from first hand. Back to Bedlam , James Blunt , Narrative 1082 Words | 3 Pages. Essays. faction or stay with them, but the essay choice all came down to essays physics, them.

THESIS: In the essay prompts novel Divergent Veronica Roth examines how the theme of choice leads to . bravery , betrayal, and greed. Body Paragraph 1: Topic sentence- Firstly the essays theme of choice leads to bravery , because of Tris selfish choice to essay, leave abnegation and go into dauntless. Essays. Point: Tris bravery in becoming a part of the dauntless is a foil to her own character, because Tris is personal narrative essay a person of action, who has courage and is brave, but Beatrice. Believe , Choice , Courage 1357 Words | 4 Pages. Winslow English II Honors 9 September 2013 The Bravery of Andy Bryant Bravery is a characteristic that, over the years, . has had much consideration. Every person has their own idea of what “ bravery ” means.

Some think of soldiers in perilous wars, or great pioneers of unconventional ideas. Legend after legend and myth after myth has been told by millions of essays people throughout history in order for each individual society to create its own idea of bravery and what the burden of being brave entails. Consequently. Believe , Courage , Ethics 764 Words | 2 Pages. also showed a lot of bravery by writing, taking the places of physics her sister in The Hunger Games this act of bravery is something that many . people would not do. If you want to be brave in your life you must be able to sacrifice yourself for another person this is personal another reason why I believe this book related to so many people. I personally enjoyed this underdog story. The fact that the least likely winner of the games came out the winner makes the physics story great to me. I admire the bravery of Katniss for putting. Believe , Love , Malnutrition 512 Words | 2 Pages.

Courage to Continue In most works of literature, a character faces a difficult feat. Atticus, Jonas, and Malala, are all courageous. The . bravery and mba admission, courage in them all is outstanding. Essays Physics. Why did they not quit as so many others had? They were scared, but faced their fears. You do not have to be strong, educated or rich to stand up for what they believe in. “True courage means doing the right thing, even in the face of seemingly impossible challenges or obstacles” In the book “To Kill a Mockingbird. Believe , Courage , Ethics 642 Words | 2 Pages. are the essay buy answers disgraceful people like Bob Ewell who is the coward. There are several events that take place in the book that show the characteristics of each of . these three characters. Atticus is the bravest person in the story. An event that shows the bravery of Atticus is when the rabid dog is walking through the physics neighborhood and about my family in spanish, he has to shoot it. Scout even says “it was times like these that my father, who hated guns, was the bravest man who ever lived.”(Pg.

134) In the case of Tom Robinson, Atticus. Essays. Core issues in ethics , Courage , Coward 585 Words | 2 Pages. Negative and Postive Effects on environment essay Movie Production. investigating on how a man was murdered and right away the figured out that the man was smothered and the killer would have to be a suffer with an essays physics, injure on reaction writing tips . his left knee. Well this scene shows how many women can be intelligent. Physics. Scenes that portray the bravery and strength of many women are shown in the beginning of the movie when saving U.S Marshall in writing tips Mongolia. They had to fight a whole army of Mongolians to save this one man. Throughout the movie these girls would always show heroic deeds in their missions.

American films , Believe , Charlie's Angels 673 Words | 2 Pages. Critic: Manifesto to Certain Filipinos. hundreds of essays years ago, despite the given joy of finally being free from Spanish tyranny. But never the less, Rizal was not shortened for his admirable deeds . that somehow it cured our fickle minds and ignorant ways. I could never forget Rizal for his bravery to have at least gave his all for essay, the country he considers more precious than his life, he was no less a true Filipino and a well-deserve hero. 2006 albums , Courage , Filipino language 468 Words | 2 Pages. resentment. In conclusion, moral values are incredibly essential for your overall well-being. Moral values offer a structure for physics, your life. Writing. Honesty . creates you respectable.

Compassion makes you sympathetic to others. Essays Physics. Courage gives you the bravery to overcome life’s challenges. Modesty keeps you focused and humble. Forgiveness allows you to become emotionally stable mainly because you don’t keep onto anger and resentment. These attributes will assist you to live your life in a way that reduces. Core issues in ethics , Courage , Feeling 576 Words | 2 Pages. connect to me with such power.

This is bullying why the poem is essays so significant, unlike any other poem; this one has a meaning which I can relate my past experiences . from one which actually bonds with me. A true meaning which I can remember forever. The bravery and hope signified by the bird is evident because it shows up in all climates, even in the storms. Personal Narrative Bullying. This shows that hope will always appear, no matter how much danger or despair torments the physics human spirit. Paper Writing Tips. Not just storm, the other hard circumstance. Emily Dickinson , Fantastic Four , Hardness 445 Words | 2 Pages. Essays. sense of being and let them forget the personal narrative about bullying unfortunate circumstances they were experiencing. ‘Bolero’ is playing majestically on essays physics the soundtrack followed by a . blackout. The blackout focuses the performance on environment essay the song; the triumphant song reflects the physics bravery of the women and environment, their resilience. Physics. The role of music in their survival is brought up again in scene seven when they recount Christmas in 1943. Bridie recounts hearing men singing near their POW camp, ‘O, Come All Ye Faithful’ is environment heard on essays the soundtrack. Audience , Barbed wire , Battery 910 Words | 2 Pages.

Elocution Piece - the Filipino Soldier by Carlos P. Essay. Romulo. Essays Physics. luminous diamond on the vast expense of Eternity. In her lies untold wealth with singing cataracts and laughing cascades coupled with golden sunsets with . Nyu Admission. myriads stars resplendent in the blue dome of the Oriental skies. But above all, there is the bravery of the Filipino Soldier. My friends behold, our dearly beloved, modest and humble – the physics Filipino Soldier. From: SELECTED ELOCUTION PIECES By: Jose Ma. Fuentes, S.J. Page 18. Carlos P. Romulo , English-language films , Incarnation 387 Words | 2 Pages. Essay. people are brave enough to stand up for something in their life. Brandon Bitner was one of the few who actually took initiative to make a stand for . Essays Physics. something he had so much trouble with. Buy Answers. This dramatic event has been heard across the physics country and the bravery of one teenager may have bettered the lives of millions. Nyu Admission. My thoughts and essays physics, prayers go out to his family and friends.

Death , High school , Intellectual giftedness 544 Words | 2 Pages. friends and after only being there for two days, she found herself back home because I was sick with the flu. Even on a normal day she will make sure that . Essay Newspaper. everything in my life is seamless, and I appreciate her so much for that. My mother has the bravery and nerve to accomplish anything, and the decency to essays, always back up her words with actions, is just a few reasons as to why she is so inspiring to me. She is constantly reminding me of my potential and never once has doubted my abilities as a child. 2006 albums , English-language films , Family 618 Words | 2 Pages. they did something on TV that was fascinating to narrative bullying, them. Even in stories, there are different types of heroes and all described differently. To me heroes are . people that save lives continuously no matter what the essays physics situations are because they have the bravery and heart to writing tips, save people.

Being a hero can lead to greatness toward people or confident gained by people. For example, in essays physics the text of “Beowulf” the Geats believed that Beowulf was going to save them from this monster named Grendel but in the film. Beowulf , Hero , KILL 656 Words | 2 Pages. pain and resentment. In conclusion, principles are extremely important for your overall well-being. principles provide a structure for your life. Honesty . Environment Essay. makes you respectable. Compassion makes you sympathetic to essays, others.

Courage gives you the bravery to overcome life’s challenges. Modesty keeps you focused and humble. Environment Essay. Forgiveness allows you to be emotionally stable because you don’t hold onto anger and resentment. These attributes will allow you to live your life in a way that reduces your stress. Core issues in ethics , Courage , Emotion 534 Words | 2 Pages. a flag represents an Ideal The unfurling of the Union Jack evokes in the English breast sentiments whose strength it is difficult to measure. The Stars and . Stripes mean a world to the Americans. The Star and the Crescent will call forth the best bravery in Islam. It will be necessary for us Indians Muslims, Christians Jews, Parsis, and all others to whom India is their home-to recognize a common flag to live and to die for. Mahatma Gandhi The Indian Flag is a national symbol and it is respected. Constitution of India , Delhi , India 4514 Words | 14 Pages.

Joey Young Dr. Essays. Wheeler Writing and Literature Studies 4-29-12 Bravery What is essay on school bravery ? Bravery is having . or displaying a sense of courage as well as being a daring individual. It is also a person who lacks the qualities of being cowardly or timid. Throughout history most great leaders showed that they were very brave individuals through their actions and as well as the essays way they carried themselves. They didn't allow what other people or forces said dictate their actions.

Leaders that show. Battle of persuasive on school newspaper Maldon , Beowulf , Byrhtnoth 1304 Words | 3 Pages. Physics. ? Bravery Bravery , a trait many men and women across the buy answers world search for; however, not many people can find . bravery in themselves. Dictionary.com defines bravery as ”brave spirit or conduct; courage; valor; or showiness; splendor; magnificence” My personal definition defines bravery as saying or doing an act to essays physics, help others even if it puts ones life is at risk. The word bravery was discovered in 1475 -1485 and the origins are Italian, “bravo”; Latin, “barbarous”; and Middle French, “brave”. The root. Coretta Scott King , Courage , Martin Luther King, Jr. 817 Words | 3 Pages. Gallantry and essay on school, other Defence awards for the personnel serving the essays physics country in either of the personal essay about bullying three forces- Army, Navy and essays, Air Forcerespectively. They are - . Common to all the paper writing Three Forces Param Vir Chakra It is the highest award for showing conspicuous bravery or pre-eminent act of valour or self sacrifice, in essays front of the enemy either on land, at sea, or in the air. It is second only to Bharat Ratna given by Government of India. The decoration was instituted on 26th Jan 1950 by the President of India.

Ashoka Chakra Award , Five-pointed star , Medal 2423 Words | 8 Pages. the Kite Runner. The definition of bravery in the Standard College Dictionary Canadian Edition is, “the quality of mind or spirit enabling one to meet danger or . opposition with fearlessness, calmness, and firmness.” Bravery is a quality that is nyu admission essay prompts shown by many of the characters in the novel The Kite Runner by Khaled Hosseini. Physics. The characters are brave in their effort to protect physical well-being, personal values, their reputation and sanity. In the novel the characters exhibit genuine bravery in order to guard the. A Thousand Splendid Suns , Fighter kite , Fremont, California 1565 Words | 4 Pages. Essay Bullying. Bravery in The Things They Carried Bravery in The Things They Carried “Success is not final, failure is not fatal: it is the courage to continue that counts.” - Winston . Churchill (Churchill) Without being courageous, you cannot be brave.

According to Merriam-Webster Dictionary, courage is “a mental or moral strength to venture, preserve, and withstand danger, fear, or difficulty.” Everyday people show bravery . Every great thing we set out to essays physics, do, whether its raise children, start a business, study for a degree, or change the nyu admission world. Army , Cold War , Courage 1305 Words | 3 Pages. Bravery , Comitatus and Pride Beowulf the 7 to 10th century poem is an epic, a long narrative poem with a serious tone, spotlighting a . heroic figure whom carries the essays physics responsibility of a nation. Beowulf is buy answers a tale of heroes, monsters and the bond of comitatus. Comitatus is defined as bond of companions, typically between a leader and his followers.

This bond has the strength to bring two warring tribes together, and has the power to win battles. This relationship is shown in essays physics more than one way throughout. Beowulf , Epic poetry , Hero 1537 Words | 4 Pages. Examination of Bravery in the Kite Runner. Examination of persuasive essay newspaper Bravery in The Kite Runner During a lifetime, most people are put in essays a situation where they can either help someone who . needs them or they can be willfully blind and not do the right thing for convenience’s sake. In the novel The Kite Runner by Khaled Hosseini, bravery is a quality that is shown by many of the main characters. These characters use bravery to nyu admission, guard the things in their lives that are important to them even if their physical wellbeing is harmed, they still stand. 2003 in literature , A Thousand Splendid Suns , Hazara people 1023 Words | 3 Pages. ?Emily Barwick Mr. Allman Senior English 16 April 20 Period 3 Bravery in Modern Times There are a lot of people that are brave today. Essays. . Being brave is to stand up against your fears, face up to nyu admission prompts, them and challenge them. Our immediate response would be to turn away from what makes us fearful and being brave enough to fight against them, without knowing the outcome, is in physics fact, an act of faith in being brave. Bravery could be found everyday from mba admission buy answers, our parents raising us (without anyone teaching them.

African American , Black people , Martin Luther King, Jr. 1037 Words | 2 Pages. Physics. Essay on Bravery and Cowardice in Private Peaceful. ?Essay on Bravery and Cowardice (Private Peaceful) In this essay I’ll be discussing the topic of bravery and cowardice and essay my family in spanish, . also stating whether or not Tommo and all the other characters are cowards or brave. Throughout Novel there are many examples of bravery and cowardice and is compared to many acts of bravery ; as a result, as soon as you’ve read an act bravery the author chose to write an act of cowardice to balance it out such as when Charlie displays courage as he protects his younger. Core issues in ethics , Courage , Coward 1100 Words | 3 Pages. Before you can actually analyze bravery and courage, you have to define it. While these two words are usually synonyms, they’re more than often . used with different meanings. For example, most people associate courage with day to day activities like confessing love, while Bravery is for life-threatening tasks. Essays Physics. In “Reno 911!” The Rock describes bravery as “When you do something dangerous, and you’re not scared”, and courage as “When you’re smart enough to know you SHOULD be scared, but you do it anyway”. Harvard University alumni , John Adams , John F. Kennedy 734 Words | 2 Pages. the way in which we wanted to essay prompts, portray the idea or thought. To me that is difficult.

I have never been one to essays physics, be short on words and can normally be found . talking to whom ever will listen. Personal Narrative Essay About. The topic that we must write on is Bravery in essays physics the face of Adversity. One definition of bravery I found was a quality of spirit that enables one to face danger or pain without showing fear. Adversity has been defined as a condition marked by misfortune or bad luck. My first thought was to write about the Civil. Believe , English-language films , Face 910 Words | 2 Pages. Bravery is a rare thing that only certain people have. Essay Prompts. Atticus Finch from the novel To Kill a Mockingbird by Harper Lee and Odysseus from . Physics. The Odyssey by Homer both show their bravery . Martin Luther King Junior one said The ultimate measure of mba admission a man is not where he stands in moments of comfort and essays, confidence but where he stands at times of challenge and controversy. In other words this quote is essay buy answers saying that a person is not necessarily a great person because of how they act every day but how. Atticus Finch , Harper Lee , Homer 478 Words | 2 Pages.

Courage and Bravery in Lord of the physics Flies. Courage and Bravery In amidst of the tragic events that occurs in essay the town of Maycomb, one positive theme that stand out throughout the whole . time is courage. Its presence is observed by essays physics, the narrator Scout from the characterisations of the central character Atticus, his influence on essay his children’s upbringing and other the members of the community that displays such qualities e.g. Essays. Mrs Dubose and Boo Radley. Atticus Finch is one of the most prominent and respected people in the town who has strong. Essay About In Spanish. Atticus Finch , Courage , Cowardice 912 Words | 2 Pages. Physics. The second day of the war in Gwalior unfortunately turned to be the last day of Rani’s life. Reaction Tips. Bravely fighting for India’ freedom, she died on June 18, 1858. . Essays Physics. Although she died in early ages, her name is essay prompts still alive on this world. Physics. She is really a bravery woman.

Baji Rao II , British Raj , Gwalior 543 Words | 2 Pages. The rich boy who had kindness and bravery hidden inside him. The Rich Boy Who Had Kindness and Bravery Hidden Inside Him The ultimate measure of a man is not where he stands in moments of comfort and . convenience, but where he stands at times of challenge.Sharon McKay’s War brothers is set in Gulu,Uganda in 2002. The book revolves around Jacob and Oteka’s life as they find themselves captured by the Lord’s Resistance Army - rebels of Uganda that abducts children to make them soldiers. Kitino Jacob is a fourteen year old that comes from a rich family, inside. Family , Kindness , Lord's Resistance Army 695 Words | 2 Pages. To Kill a Mocking Bird Bravery Theme. To Kill a Mocking Bird Essay Jumping off a cliff, shooting someone, or fighting are just some examples of what are considered brave. Essay. Although in essays physics To Kill a . Persuasive Newspaper. Mockingbird, Harper Lee gives a different view of essays physics real courage as one of her themes and mba admission, what it means to physics, be brave.

She shows it through many of her characters like Atticus, the wise lawyer and Mrs. Dubose, a rapacious old lady. She even has the quietest of the characters show courage in his actions. About. Courage is not being able to physics, shoot, but to. Atticus Finch , Harper Lee , Monroeville, Alabama 810 Words | 2 Pages. spoken by George Sewell (Famous 5), properly abridges the theme of bravery in personal narrative about Harper Lee's To Kill a Mockingbird. While the coward of the essays physics novel . dies in nyu admission essay the end, the brave ones who oppose him end up alive. Even though it seems at essays physics times that those characters which represent the theme in its most honorable form are losing, this is not the case. They face more hardships, but nobility prevails in on school the end when it comes to life and death. Bravery is an important theme all throughout To Kill a Mockingbird, and.

Atticus Finch , Courage , Cowardice 952 Words | 3 Pages. Essays. Class VIII 1. Find the smallest number by which 9408 must be divided so that it becomes a perfect square. Also, find the square root of the perfect square . so obtained. Essay. Ans: 56 2. 5929 students are sitting in essays physics an auditorium in such a manner that there are as many students in a row as there are rows in the auditorium. How many rows are there in the auditorium? Ans: 77 3. Find the square root of ( i ) ( ii ) 4 ( iii ) 23 ( iv ) 5.774409 ( v ) 0.00053361 Ans: 25/36;. Decimal , Division , Exponentiation 390 Words | 2 Pages. Navy SEAL Ralph on the Battlefield.

Responsibility A. Making sure everyone is okay B. Ensuring team is safe II. Leadership A. Ideas for help . B. Tactics in enemy territory III. Bravery A. The beast B. The missions Conclusion- Ralph’s responsibility, leadership, and bravery were apparent through his actions on the island; he would make a great Navy SEAL. Anna Mrs. Conti English II Honors 27 November 2013 Navy SEAL Ralph on the Battlefield . Essay About In Spanish. Coronado, California , Operation Red Wing , Special Activities Division 1280 Words | 5 Pages. a hero, but it is essays physics not necessary one should be rich, handsome and or educated to be a hero.

What is persuasive on school hero and physics, what makes person a hero? Heroes are role models . of personal bullying society because they have several important characteristics such as living for ideals, bravery , skill and talent. Heroes believe in essays and follow ideals. Heroes have principles to serve individuals and society as well. Their desire to achieve a goal is different from other people. In the article, Is a Hero Really Nothing But a Sandwich? Ted. British Empire , Courage , Hero 888 Words | 3 Pages. Persuasive Essay On School Newspaper. theme dealt with courage and bravery . e. According to the way Harper Lee wrote her book, she also thinks that courage and . bravery were also a very important theme of the essays physics novel. To prove this, I will show evidence from the persuasive newspaper book like: when Scout and Jem stood up to the lynching mob, when Atticus decided to stand up against the side of the whites because he knew Tom was innocent, and also when Mrs.

Dubose stopped her drug addiction. II. Essays. a. One of the reasons why bravery was a main part of the about book. Addiction , Alcoholism , Drug addiction 1206 Words | 4 Pages. heroic tale of a man#8217;s strength pitted against essays forces that he can#8217;t control. It is a tale about an old Cuban fisherman and his . three-day battle with a giant Marlin. Environment Essay. Through the use of three prominent themes: friendship, bravery , and Christianity. The Old Man and the Sea strives to teach important life lessons to the reader. One of the most important themes in physics this story and in life is, friendship. The relationship between the old man and the boy is.

Boy , Ernest Hemingway , Joe DiMaggio 1256 Words | 5 Pages. amp; The Minor Canon” amp; Stone’s “Where I Find My Heroes” provide what are the qualities of a hero. Also does Mariah Carey’s “Hero” and Bulfinch’s “The . Quest of the golden fleece”. Reaction Tips. The qualities of essays physics a great hero are bravery , courage, amp; strongness. Nyu Admission Prompts. An excellent hero needs bravery to accomplish things they want to succeed in. For example, “A Hero comes along with the strength to carry on essays and you cast your fears aside and you know you can survive”(Mariah Carey “Hero”1). This quote proves if you. Anxiety , Courage , Hero 920 Words | 3 Pages. Reaction Paper Writing. 2013 Bravery and Courage Through mysterious adventures and horrific events there is bravery and courage shown in essays To Kill a . Mockingbird.

The novel, published in 1960, is very well written. Essay About Bullying. The author, Harper Lee, used some of essays her experiences as a child. Persuasive Newspaper. This book displays many actions of bravery and courage. Characters in this novel that display bravery and essays physics, courage are Atticus, Mrs. Paper Tips. Dubose, and Boo Radley. True bravery and essays physics, courage is hard to find in people. Atticus displays true bravery and courage. Atticus Finch , Harper Lee , In Cold Blood 1056 Words | 2 Pages. RUNNER AND TSOTSI In the novel, The Kite Runner written by Khaled Hosseini and the film Tsotsi written by environment, Athol Furgard, many similarities can be found. Essays. . Both stories include the inner battle that people face throughout their life, the meaning of bravery and what brotherhood truly represents.

In today's society, people find it difficult to perceive their values and what they believe in. The inner battle isn't the only concept that the novel and the movie share, but many more parallel ideas will be. A Thousand Splendid Suns , Hazara people , Khaled Hosseini 1257 Words | 4 Pages. Maram Anabtawi Exam 2 Folklore and Fairytale In The Hobbit, there are major themes of bravery and transformation. The main character goes . through a lot of character and personality changes. By the end of the novel the hobbit, also known as Bilbo Baggins, is mba admission almost a different person with many new characteristics. Bilbo Baggins starts out as a round bellied little creature who is happy staying at essays home. Reaction Writing Tips. Bilbo is half the size of a man and likes comfortable, peaceful life. Bilbo has two family.

Bilbo Baggins , Gandalf , Gollum 1018 Words | 3 Pages. line there are a number of positive themes presented to the reader throughout the story, e.g. education, bravery and essays, growing up. The theme . of bravery is persuasive essay on school amplified by the characters of essays physics Atticus and Jem. Atticus is the essay moral voice of the novel and his decision to defend Tom Robinson even though he knows there would be controversy and attacks on his character and family establishes his bravery . Early in the novel Jem learns that Atticus' disapproval of guns and Atticus's opinion that guns don't make. Atticus Finch , Great Depression , Harper Lee 989 Words | 3 Pages. Marshall uses stories he has attained throughout his lifetime, mostly from his grandfather, to emphasize the importance of physics twelve main facets of life. These . twelve facets are: humility, perseverance, respect, honor, love, sacrifice, truth, compassion, bravery , fortitude, generosity, and wisdom. Throughout each chapter pertaining to these traits, Marshall uses his stories to apply them to everyday life and to gain a sense of respect for his elders. In his introduction, Marshall states, “The stories I heard. Compilation albums , Courage , Lakota people 889 Words | 3 Pages. harder. That’s right, bravery . Prompts. Even though heroes have at essays least one common trait, depending on the time period, the media depicts heroes to mba admission buy answers, the . public eyes.

In the various cultures that exist, they inhabit different stories. Each of these stories depicts a hero in some shape or form which influences the creation of other heroes. Heroes contain commonalities in different ways by essays, being defined across various cultures and time periods. Reaction Writing. Each hero has a common trait which is bravery . Bravery’s definition. Essays. Courage , Definition , Hero 921 Words | 3 Pages. but he had fled; The flame that lit the battle’s wreck Shone round him o’er the dead. Reference to narrative, Context: These lines have been taken from the poem . “Casabianca” written by Felicia Dorothea Hemans. In the poem poetess conveys the message of bravery , sacrifice, responsibility, patriotism and discipline through the story of Casabianca. Explanation: In these lines poetess tells that Casabianca was standing on the board of the ship which was on fire. Essays Physics. He was standing alone while all others had.

Casabianca , Felicia Hemans , Lydia Sigourney 1457 Words | 6 Pages. What Makes Beowulf a Hero and How Does It Compare to Modern Day Heros. thinks of a hero, bravery is often a common quality, but there are many examples of bravery . good TS In Beowulf, he displays . countless actions of bravery , but one that really stands out in the text is when he is old and has to face the dragon that is terrorisufficatingzing his kingdom; “I won’t shift a foot when I meet the cave-guard: what occurs on the walls between the reaction tips two of us will turn out as fate, overseer of men, decides” (283-286). Martin Luther King Jr. displayed bravery in a different way. African American , Courage , Hero 851 Words | 3 Pages. Physics. Germanic Tribal Characterisitcs of Beowulf. leadership. While many might consider Beowulf a proud and arrogant man, he was a hero to the Germanic tribes. The character of Beowulf embodies the very . values and ideals of what a hero was to essay about my family in spanish, the Germanic peoples. Through his respect to kinship, bravery , honor, strength, and intelligence, Beowulf offers to Germanic men a model of a great warrior and leader, a model every man should strive to emulate. Early Germanic social organization revolved first and foremost around kin and family, the physics first. Anglo-Saxons , Beowulf , Germanic paganism 893 Words | 3 Pages.

The Old Man and the Sea is reaction paper writing a heroic tale of mans strength pitted against essays forces he cannot control. It is a tale about an mba admission essay, old Cuban fisherman and his . three-day battle with a giant Marlin. Through the use of three prominent themes; friendship, bravery , and physics, Christianity; the Old Man and personal essay about, the Sea strives to teach important life lessons to the reader. The relationship between the old man and the boy is introduced early in the story. They are unlikely companions; one is old and the other young, yet. Boy , Ernest Hemingway , Jesus 955 Words | 3 Pages.

It Feels a Shame to Be Alive (Dickinson) - Analysis Essay. questions the bravery of the essays living. Despite death being a part of persuasive essay day-to-day life during a time of war, death was an unknown state of being . and for this reason it scared people and the topic was avoided, as it still is today. This poem questions the bravery of those who live “When Men so brave – are dead”; this was the unmentionable subject during the American Civil War. Essays Physics. In that time a man’s honor and bravery was more important than his life, and to persuasive on school newspaper, question the honor and bravery of physics a man who survived. American Civil War , Civil war , Emily Dickinson 863 Words | 3 Pages. Romeo and Juliet: How Shakespeare Held the Audience's Attention. inspires them to re-evaluate their presuppositions, and learn to denote an emotional understanding of someone else’s feelings or problems by walking around . in mba admission essay buy answers their shoes. Themes concerning injustice of racial prejudice and the value of courage and bravery are traversed in great depth through the main characters, Atticus and Scout Finch, and their correlations with others in turn allow the reader to essays, relate the lessons learnt to buy answers, their own lives. One of Lee’s most profound themes is the essays injustice of. African American , Black people , Harper Lee 1187 Words | 5 Pages. comparing and contrasting Odysseus and Percy Jackson. place in ancient Greek culture, and Percy Jackson, the main character in the novel The Lightning Thief, likenesses and differences within their character . Reaction Paper Writing Tips. traits are easily able to be recognized.

Although Odysseus and Percy both exhibited loyalty and bravery , the characteristic of essays confidence was not equally shared between them. Odysseus and Percy had a lot in common, especially a strong, passionate love towards their families to whom they treated with utmost loyalty. Newspaper. The two characters were both separated. Bronze Age , Confidence , Homer 879 Words | 4 Pages. all of his subjects, and Malcolm has the potential to be a great king through the way in which he fights to overthrow Macbeth. Physics. Macbeth, however, through his . lack of noble qualities is another story. Through arrogance, actions, and cowardice versus bravery Macbeth emerges as the only true failure as a king. Arrogance surfaces in Macbeth because the protagonist believes that he knows better about everything than anyone else.

For example, Macbeth is arrogant to his own thoughts and feelings. He easily. Reaction Tips. Core issues in ethics , Courage , Cowardice 1086 Words | 3 Pages. Crusades; because of his bravery , England is saved. Glory and Bravery are not the essays physics only things Ivanhoe is demonstrating by . fighting for England. He is also showing a fear of disgrace. If his home country of environment England were to essays, lose the environment essay battles, he and his country would be disgraced. His fear of disgrace helps to lead him on.

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It would be the best if I could copy my introduction from resume to CL, because there is almost the same to write. Physics? please advise. Some of the personal narrative about contents from physics your resume intro should be used in you CL, but should not simply be copied. If you read the above guide and download the template pack, you will learn exactly what information should be included. Also, be sure to check out persuasive essay on school newspaper our CL samples to essays physics, give you a better idea on how you can word your cover letter. Good luck on the job hunt! I am trying to write a cover letter for nyu admission essay a managerial position in the hospitality field. However, I do not see any examples. We are in the process of adding new cover letter samples and essays physics, have included your suggestion in the list. In the meantime, follow the guide above and download one of cover letter templates. Good luck on the job hunt! Basically, a cover letter is a letter of essay in spanish introduction.

Usually I state the essays physics name of the position I’m applying for and why I am qualified for the position. At the end of the letter, I’ll write something like “If you have any questions or to schedule an essay, appointment for an interview, please contact me at ***-**-****.” Then double space and type “Sincerely” and then space 4 times and type your name. You’ll be signing in the space above your typed name. This has been most helpful. GBU. Glad you liked it.

Share it with you your friends. GBU, too! I am working on a resume for essays physics an experienced welder, One of the requirements is a valid drivers license, which I do not possess at this time , but if I was hired, I could pay the traffic fine and personal narrative essay bullying, get my license back with my first paycheck. Should I explain this on my resume or by essays physics, telephone or in person or not at mall. In all honesty, your best bet is to essay about my family, find a way to pay the traffic fine and essays physics, get you license back before applying for the position. Thanks for the advice. I was thinking that very thing or appearing at traffic court and setting up a payment plan which would clear the way for a re issue. All about the money. which I happen to environment essay, be in short supply of. Essays? Thanks again. We know the struggle.

Hang in environment essay, there brother! Try add figures that will impress an physics, employer and that pertain to the position you are applying for. Nyu Admission Prompts? Best of luck! I live in Thailand and odd numbers here are auspicious so it probably won’t hurt to throw some of them in. I’m still not sure what you all are referring to with bullet point ‘numbers’. Can someone explain? Thank you. thank you so much RG you really shade light on essays physics cover letters, i was clueless wat it was so mostly i would ignore it and essay, send my applications without one from today i will do thngs differently. Download our cover letter template pack and essays physics, use the tips written above.

That should get you started. Best of narrative bullying luck! How to address gaps in employment. My mom passed away unexpectedly in 2005. I was incapacitated by depression and unable to work from 2005-2008. Got a job in 2008 was employed until 2012 when I had a baby. i have been a stay at home mom. Ready to go back to work again. How do I address the gaps when posting my resume on job resource sites? I have spent the past six months working reworking my resume. Essays? I’ve searched hundreds of resume websites; Resme Genius is, by far, the best.

Would appreciate any advice. Thanks for writing tips the kind words. One of the easiest ways to essays, mask your work gaps is to use a functional format: https://resumegenius.com/resume-formats/functional-samples-writing-guide. Give it a try and see what you think. Good luck on the job hunt! How do explain a reason for leaving a company when you hate your job/employer, more management than the job itself. We always recommend not to burn any bridges with former employers and to leave on a positive note. So in essay, that case, we suggest that you explain that you are looking for an opportunity in a new industry or a different position.

Although it may sting, make sure to also thank management for the opportunity you were given. Good luck! Struggling with how to address a cover letter for a summer internship. I’m not applying for a posted internship, just going to show up at an organization that my school advisor suggested I reach out to. I have no idea who I’ll be giving my cover letter to, but I don’t want to say “dear hiring manager” because technically they wouldn’t be hiring me… right? Aside from essays that, this guide was still very helpful. We’re glad that you found our advice helpful. In your case, “Dear Hiring Manager” or “Dear Madam or Sir” would be appropriate.

Good luck! So i have been asked to email my salary requirements, Here is my situation though, I previously worked with this company in the same exact role. I left for another job opportunity ( which built my skill set and experience) about a year ago this month. They have asked me to come back and fill that position again. How do I compose a salary requirement. In this situation you have the leverage, so it would be fair to ask for 10-20% more than you were paid before. The skills and narrative, experience you gained over essays physics the last year make you more valuable than you were before. Good luck! Hi, I am trying to help a friend who is looking to move into a completely different line of work, he has only had 1 job since high school, which has nothing to do with what he really wants to do. How do you approach that in a cover letter, since he has no experience in the field? Your friend will have to discuss their general skills or traits that are relevant to essay on school, the job.

I just turned 62 and want to retire from my current job, but not ready to retire. Any suggestions on how to “put my best foot forward” when applying for a new position in an industry I’ve never worked in before? When applying for a position in an industry that you’ve never worked in physics, before, the first thing you should do is come up with a list of the relevant skills that you can transfer over to that industry. From there, you can work on ‘selling’ those particular skills in your cover letter and resume. Good luck! So glad I stumbled upon this. Glad you found it helpful! I’m applying for a new job but the mining company has different projects.

Would I have the company’s address or the project site address? We recommend that you use the address that the cover letter is being sent to. If you are not sure, then use the company’s address. Good luck! Some cover letter here have helped me move on to another level. Through this i had to pay bills for my ailing child . About My Family? Thanks you have been of essays physics great help through the way to write a winning cover letter. Great information! My question is I have been out of the work force for about a year and a half due to birthing children and staying home for mba admission essay them. I am wondering if this is something i should include on my CL to explain my gap in work history or is this information to personal to include? Great question!

If your work gap is physics, longer than a couple of months, then you should certainly include an essay about my family in spanish, explanation in your cover letter. Don’t spend more than 1-2 sentences on your explanation though. Remember that you want to keep the focus of your cover letter on your experience and qualifications. You can say something like “After taking some time at home with my family,” then express how excited and enthusiastic you are to return to the work force and physics, how this position is exactly what convinced you that now is the time to do so. You can use that phrase for essay prompts maternity leave, or caring for older family members, or even your own issues. Essays Physics? Shoot, you can use that even when you didn’t choose to take a break, so to speak. Good luck!

I’m a college dropout and I couldn’t decide on which template to use for my cover letter. Essay? I quit school during my sophomore year. We suggest using the ‘Non-Student’ templates. If your college experience is relevant to the job you are applying for, you might consider including it your cover letter. Best of luck on the job hunt! Thank you Resume Genius! what if there is no job description given? how do you start of with the physics cover application in such a scenario? Even without a job description you can still follow the template above. Look up job descriptions for essay about my family in spanish the same position from other companies to essays physics, get an nyu admission prompts, idea of essays what the employers is looking for. Unless requested in the job ad, leave your salary requirements off your cover letter.

Good Luck! Fair point – it’s always good to be careful when sending out personal information. Thanks for the suggestion! Share How to Write a Cover Letter #038; 40+ Free Templates Our code geeks and HR experts are proud to introduce our new Free Resume Builder software to help you land more interviews in today’s competitive job market. We provide HR-approved resume templates, built-in job description bullet point phrases to choose from, and easy export to MS Word and essay, PDF. Get awesome job opportunities sent directly to your inbox. By clicking Send Me Job Alerts, I agree to the Resume Genius Terms of Use and Privacy Policy. Play the One-Minute Game That’ll Show You How to Improve Your Resume. Think you can judge the essays physics quality of a resume within 6 seconds? The answer may surprise you.

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Transcriptomic analysis of the autophagy machinery in crustaceans. The giant freshwater prawn, Macrobrachium rosenbergii , is a decapod crustacean that is commercially important as a food source. Farming of commercial crustaceans requires an efficient management strategy because the animals are easily subjected to stress and diseases during the culture. Autophagy, a stress response process, is well-documented and conserved in most animals, yet it is poorly studied in crustaceans. In this study, we have performed an in silico search for essays physics, transcripts encoding autophagy-related (Atg) proteins within various tissue transcriptomes of M. Environment Essay. rosenbergii . Basic Local Alignment Search Tool (BLAST) search using previously known Atg proteins as queries revealed 41 transcripts encoding homologous M. rosenbergii Atg proteins. Among these Atg proteins, we selected commonly used autophagy markers, including Beclin 1, vacuolar protein sorting (Vps) 34, microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B), p62/sequestosome 1 (SQSTM1), and lysosomal-associated membrane protein 1 (Lamp-1) for further sequence analyses using comparative alignment and protein structural prediction. We found that crustacean autophagy marker proteins contain conserved motifs typical of other animal Atg proteins. Western blotting using commercial antibodies raised against human Atg marker proteins indicated their presence in essays, various M. rosenbergii tissues, while immunohistochemistry localized Atg marker proteins within ovarian tissue, specifically late stage oocytes. This study demonstrates that the molecular components of autophagic process are conserved in essay about my family in spanish, crustaceans, which is comparable to autophagic process in mammals. Physics. Furthermore, it provides a foundation for further studies of essay buy answers autophagy in crustaceans that may lead to essays, more understanding of the reproduction- and stress-related autophagy, which will enable the about my family, efficient aquaculture practices.

Electronic supplementary material. The online version of this article (doi: 10.1186/s12864-016-2996-4 ) contains supplementary material, which is available to authorized users. Macroautophagy (autophagy) is a complex process involving degradation of cellular constituents to essays physics, maintain organelle and protein homeostasis in reaction, response to various stresses, including genomic, oxidative, hypoxic, proteotoxic and essays physics, metabolic stresses as well as starvation [ 1 ]. In Spanish. Autophagy plays a major role in the removal of mutated or oxidized protein aggregates and of damaged organelles. It is also involved in restoring nutritional and metabolic imbalances during starvation. Deficiency in physics, the clearing and essay prompts, restoring function of autophagy leads to cell death, which ultimately contributes to growth retardation and various degenerative diseases, including neurodegenerative, muscular and essays physics, cardiovascular diseases [ 2 , 3 , 4 , 5 ].

Autophagy is an evolutionary conserved mechanism ubiquitously present in environment, all eukaryotic cells. It is physics driven and about my family, regulated by physics several proteins designated as autophagy-related (Atg) proteins (ATG in mammals). At least 32 Atg genes have so far been identified in the yeast, Saccharomyces cerevisiae , the species initially employed for genetic screening of autophagy [ 6 ]. Many Atg orthologs have subsequently been identified in higher eukaryotes, including nematodes ( Caenorhabditis elegans ) [ 7 ], arthropods ( Drosophila ) [ 8 ], mammals (mouse and human) [ 9 ], as well as in plants [ 10 ]. Furthermore, these Atg genes seem to be highly conserved among different metazoan phyla. Amongst these, phylum Arthropoda has the essay buy answers, largest number of species and their members are widespread throughout both terrestrial and aquatic habitats. While autophagy in insects has been studied intensively [ 8 , 11 , 12 , 13 , 14 , 15 ], the autophagic machinery in crustaceans is still unexplored. Essays. However, a few studies have shown the presence of autophagosome within the cells of the midgut in crustacean species by using electron microscopy and it has been suggested that autophagy might play an important role for midgut cell survival [ 16 , 17 , 18 , 19 ]. Many species of crustaceans, especially decapods (shrimps and crabs), are a commercially important food source. During captivity in farming, these animals can be subjected to many stresses that affect feeding, growth, reproduction, and diseases.

Hence, management of buy answers stress, disease, and essays, reproductive manipulation are crucial in farming. Since these processes are related to reaction, autophagy [ 20 , 21 , 22 ], an understanding of the autophagic process in crustaceans is essays important for mba admission essay buy answers, improvement of crustacean aquaculture. To date, the genome of the water flea, Daphnia pulex , had been generated [ 23 ]. Further, the transcriptomes of several crustaceans, including the mud crab ( Scylla olivacea ) [ 24 ] and white shrimp ( Litopenaeus vannamei ) [ 25 ], have been provided in essays, the online databases. Reaction Writing. Recently, we have generated transcriptomes from the eyestalk, central nervous system (CNS) and ovaries of M. rosenbergii , leading to the identification of several genes encoding neuropeptides [ 26 , 27 ] and steroidogenesis-related proteins [ 28 ]. In this study, we identified Atg genes in the above-mentioned M. rosenbergii transcriptomes and then examined the presence of key Atg proteins in the tissues using Western blotting and immunohistochemistry. Furthermore, structural comparisons between human and M. rosenbergii marker proteins for autophagic process, including Beclin 1 (BECN1), vacuolar protein sorting (Vps) 34, microtubule-associated proteins 1A/1B light chain 3 (MAP1LC3 or LC3), p62/sequestosome 1 (SQSTM1) and lysosomal-associated membrane protein 1 (Lamp-1), were performed to show that crustacean Atg marker proteins have a similar structure, and physics, possibly functional activity, to those of environment human. In summary, our findings provide a substantial evidence for the presence of autophagy machinery in a crustacean species. Gene mining of autophagy-related proteins in M. rosenbergii transcriptomes.

Atg transcripts present in M. rosenbergii transcriptomes in comparison with Atg genes of mammals and yeast. Autophagy core machinary. Number of hit transcript in M. rosenbergii transcriptomes. ULK1 protein kinase complex. 1 (same hit with ULK2) WIPI-1,2,3,4 Vps34-beclin1 class III PI3-kinase complex.

1 (same hit with GABARAP) ATG4A-D (autophagins 1–4) beclin 1 associated autophagy related key regulator like. The number of predicted Atg transcripts derived from M. rosenbergii transcriptomes is indicated. Sequence analyses of Atg marker proteins in M. Physics. rosenbergii. Identification of an M. rosenbergii Atg6. a A schematic annotation of M. rosenbergii Atg6 (MroAtg6) and the alignment of personal narrative about bullying crustacean Atg6 proteins with Atg6/BECN1 of other species from different phyla. Essays. Black shading indicates conserved amino acids while grey shading indicates similar amino acids. Green bar indicates the region of Bcl-2 homology 3 (BH3) domain of my family in spanish HsaBECN1. Mro, Macrobrachium rosenbergii ; Ple, Pontastacus leptodactylus ; Cfi, Calanus finmarchicus ; Dpu, Daphnia pulex ; Dme, Drosophila melanogaster ; Cgi, Crassostrea gigas ; Spu, Strongylocentrotus purpuratus ; Cel, Caenorhabditis elegans ; Dre, Danio rerio ; Xtr, Xenopus tropicalis ; Mmu, Mus musculus ; Hsa, Homo sapiens . b Predicted tertiary structures of HsaBECN1 ( a ) and essays, MroAtg6 ( b ) and superimposition of HsaBECN1 and MroAtg6 ( c ). The BH3 domain is labeled on HsaBECN1, corresponding to amino acid positions 105–129. 65–76 % similarity to the human ortholog (HsaVps34).

Conserved functional domains, including C2 (positions 27–183; Pfam accession number: PF00792), phosphatidylinositol 3-kinase (PI3K) accessory (PI3Ka; positions 280–539; Pfam accession number: PF00613), and PI3K catalytic (PI3Kc; positions 576–924; Pfam accession number: PF00454) domains could be annotated in MroVps34 (Fig. 2a ). A high degree of essay amino acid conservation was found throughout the protein length, especially within C2 and PI3Kc domains (Fig. 2a ). Physics. Structural superimposition of HsaVps34 and MroVps34 shows similarity in folding between these two proteins (Fig. 2b ). Identification of an M. rosenbergii Vps34. Essay. a A schematic annotation of M. Essays. rosenbergii Vps34 (MroVps34) and the alignment of Vps34 in crustaceans and other species from environment, different phyla (only the alignments of essays physics amino acids within C2 and phosphatidylinositol 3-kinase catalytic (PI3Kc) domains are shown). Black shading indicates conserved amino acids while grey shading indicates similar amino acids. Mro, Macrobrachium rosenbergii ; Ple, Pontastacus leptodactylus ; Pmo, Penaeus monodon ; Dpu, Daphnia pulex ; Dme, Drosophila melanogaster ; Cel, Caenorhabditis elegans ; Dre, Danio rerio ; Xtr, Xenopus tropicalis ; Rno, Rattus norvegicus ; Hsa, Homo sapiens ; Sce, Saccharomyces cerevisiae . b Predicted tertiary structures of HsaVps34 ( a ) and MroVps34 ( b ) and superimposition of HsaVps34 and MroVps34 ( c ). Buy Answers. The C2 (amino acid positions 26–186) and PI3Kc (amino acid positions 539–885) domains of essays HsaVps34 are labeled in personal narrative essay about bullying, red and green colors, respectively. 60 %, Fig. 3a ). The MroAtg8 consists of 122 amino acids, with 98 % identity to M. nipponense Atg8. An Atg8 ubiquitin-like domain (Pfam accession number: PF02991) is present within MroAtg8 (amino acid positions 16–121, Fig.

3a ). Essays. The MroAtg8 and human MAP1LC3B (HsaMAP1LC3B) share 72 % similarity, and essay, their structural superimposition indicates a similar secondary structure, including at physics, the binding sites for Atg7 and tubulin, as shown in HsaMAP1LC3B (Fig. 3b ). The amino acid residues which are responsible for the interaction between Atg8/MAP1LC3B and other autophagy receptors [ 32 ], and the amino acid residues which are important for the formation of two binding pockets are shown in reaction, Fig. Physics. 3a . Mba Admission Buy Answers. We could observe that those important residues were also conserved in physics, crustacean Atg8. Therefore, the basic residues important for electrostatic interaction of MroAtg8 with other proteins could be predicted, possibly including R 11 , R 12 , K 31 , K 50 , K 52 , and R 71 (Fig. Mba Admission Essay Buy Answers. 3a ). Physics. Likewise, the narrative, residues required for essays, the formation of two binding pockets in MroAtg8 could be predicted (D 20 , I 24 , P 33 , I 35 , K 52 , L 54 , and F 109 for the formation of the first pocket and I 36 , F 53 , V 55 , P 56 , V 59 , L 64 , I 67 , I 68 , and R 71 for the formation of the second pocket) and essay prompts, also mapped in the predicted MroAtg8 tertiary structure (yellow and essays physics, pink areas, respectively; Fig. 3c ), assembling the appearance of two adjacent pockets. Identification of an prompts, M. rosenbergii Atg8. a A schematic annotation of essays M. rosenbergii Atg8 (MroAtg8) and the alignment of about crustacean Atg8 proteins with Atg8/LC3 of physics other species from different phyla. Black shading indicates conserved amino acids while grey shading indicates similar amino acids. Mba Admission. Blue boxes indicate the basic residues which are previously known to be critical for electrostatic interaction of LC3 with other proteins. Yellow and pink bars underneath the alignment indicate amino acids constituting two hydrophobic pockets of HsaLC3 (each color represents each pocket) [ 32 ]. Physics. Mro, Macrobrachium rosenbergii ; Mni, Macrobrachium nipponense ; Lva, Litopenaeus vannamei ; Sol, Scylla olivacea ; Esi, Eriocheir sinensis ; Dpu, Daphnia pulex ; Dme, Drosophila melanogaster ; Cel, Caenorhabditis elegans ; Dre, Danio rerio ; Xtr, Xenopus tropicalis ; Mmu, Mus musculus ; Hsa, Homo sapiens . b Predicted tertiary structures of HsaLC3 ( a ) and personal narrative essay bullying, MroAtg8 ( b ) and superimposition of HsaLC3 and MroAtg8 ( c ). Essays. The binding sites for Atg7 (red; amino acid positions 38, 82, and 114) and tubulin (green; amino acid positions 8, 17, 21, 22, 24, 25) in HsaMAP1LC3B are indicated. c Predicted MroAtg8 tertiary structure with hydrophobic surface.

Residues which were predicted to environment essay, form two hydrophobic pockets in MroAtg8 are colored in yellow and pink. Identification of an essays physics, M. rosenbergii p62. a A schematic annotation of M. rosenbergii p62 (Mrop62) and the sequence alignment of crustacean p62 proteins with p62 of personal about bullying other species from essays, different phyla. Black shading indicates conserved amino acids while grey shading indicates similar amino acids. Mro, Macrobrachium rosenbergii ; Ple, Pontastacus leptodactylus ; Dpu, Daphnia pulex ; Dre, Danio rerio ; Xtr, Xenopus tropicalis ; Mmu, Mus musculus ; Hsa, Homo sapiens ; Ame, Apis mellifera ; Spu, Strongylocentrotus purpuratus ; Gga, Gallus gallus ; Cfa, Canis familiaris . Mba Admission. b Local alignment of LIR domain sequences of predicted Mrop62 and p62 of other species. c Predicted tertiary structure of Mrop62. The predicted Phox and Bem1p (PB1; amino acid positions 6–96), ubiquitin-associated (UBA; amino acid positions 588–634), and LC3-interacting region (LIR; amino acid positions 534–555) domains of Mrop62 are colored in yellow, purple, and red, respectively. Identification of an physics, M. rosenbergii Lamp-1. A schematic annotation of M. Essay In Spanish. rosenbergii Lamp-1 and the alignment of crustacean Lamp-1 proteins with Lamp-1 of essays other species from different phyla are presented. Persuasive. A signal peptide (blue), a mature peptide (green), a conserved transmembrane domain of the epidermal growth factor receptor (TM-EGFR) (yellow), and cysteine residues are indicated. Black shading indicates conserved amino acids while grey shading indicates similar amino acids. Mro, Macrobrachium rosenbergii ; Mni, Macrobrachium nipponense ; Ple, Pontastacus leptodactylus ; Dpu, Daphnia pulex ; Dme, Drosophila melanogaster ; Cgi, Crassostrea gigas ; Dre, Danio rerio ; Xle, Xenopus laevis ; Rno, Rattus norvegicus ; Hsa, Homo sapiens.

Spatial expression of autophagy markers. Validation of the autophagy marker proteins in female M. rosenbergii tissues. Western blotting shows the expression of Atg6/BECN1, Atg8/LC3, and physics, p62/SQSTM1 in mba admission buy answers, various tissues, including the hepatopancreas (Hp), ovary (Ov), muscle (Ms), brain (Br), eyestalk (Es), and thoracic ganglia (Tg). Physics. The expression of these marker proteins in human squamous carcinoma cells (A431 cell line) is used as a positive control. The amount of total protein loaded per lane was 30 ?g. Immunoperoxidase staining of essay about in spanish Atg marker proteins in the ovary of M. rosenbergii . Late vitellogenic oocyte (Oc4) with HE stain ( A ). Immunolocalization of Atg6/BECN1 ( B ), Atg8/LC3 ( C ), p62/SQSTM1 ( D ), and Lamp-1 ( E ) in physics, the Oc4 of ovarian sections. The insets ( b – e ) show images at a higher magnification where positive staining is visible as red spots (arrows). F Negative control. Scale bars: 25 ?m ( A and F ) and 10 ?m ( B – E ). (N, nucleus; Yg, yolk granules; Li, lipid droplets; Mb, cell membrane)

Autophagy refers to a lysosome-driven process for the degradation of essay my family in spanish cellular components that are redundant, aged, or damaged [ 37 ]. The control and physics, execution of autophagy have been well characterized in the yeast S. cerevisiae [ 38 ] and the worm C. elegans [ 39 ], and are being investigated in narrative about bullying, human, mouse, the fruit fly ( Drosophila melanogaster ), and physics, plants. Here we present the first study showing that the key genes and proteins involved in personal narrative essay, the regulation and physics, execution of autophagy are present in crustaceans. All Atg proteins investigated could be identified from M. rosenbergii transcriptomes (see Table 1 ), confirming their widespread conservation amongst animal phyla. It is persuasive newspaper likely that the autophagic process in crustaceans follows the same pathway as in essays physics, other animals, engaging a similar set of proteins from the essay, initial triggering step (the activation of the essays, ULK1 protein kinase complex) until the formation of environment essay active autophagic vesicles (the autolysosomes). The same hit transcripts were observed for GABARAP and GABARAPL1, and ULK-1 and ?2 transcripts. In the case of GABARAP and GABARAPL1, it has been shown that there are at essays physics, least three human GABARAPLs (1–3) genes which are homologues of the GABARAP gene [ 40 ]. Therefore, GABARAP and GABAAPL1 share a high sequence similarity and both, as well as other GABARAPLs, are categorized within the newspaper, GABARAP family [ 41 ]. The result from our transcriptome analysis suggested that only a single transcript encoding MroGABARAP exists in the prawn, possibly because other homologous genes may have been lost during evolution. Atg6 (Vps30 in yeast) was initially isolated as an interactor of the anti-apoptotic protein Bcl-2, and thereafter named Beclin 1 [ 42 ]. Physics. Atg6/BECN1 was next shown to be an essential activator of personal essay about bullying Vps34 for the initiation of the autophagic process [ 43 ]. High conservation of protein amino acid sequence, especially at essays, the C-terminal region, could be observed in this protein among vertebrates and tips, invertebrates, indicating that those conserved motifs are significant for the activity of physics this protein. In particular, amino acid conservation within the persuasive essay on school, BH3 domain region and structural superimposition of human BECN1 and M. rosenbergii Atg6 indicates that M. rosenbergii and other crustacean Atg6 may interact with Bcl-2, and essays physics, thereby play a regulatory role in apoptosis.

Upon interaction with BECN1, the PI3K class III protein (PI3KC3 in essay about, human or Vps34 in yeast) catalyzes the formation of physics PI3P, which is essential for the recruitment of autophagosomal membranes [ 44 , 45 ]. Vps34 protein is the most evolutionarily conserved protein within the PI3 kinase family [ 46 , 47 ]. We did consistently find that crustacean Vps34 showed high conservation in buy answers, their amino acid sequences with those of other invertebrates and physics, vertebrates, especially at the domains described as critical for function. My Family. In fact, the Vps34 protein contains three major domains, namely the C2, PI3Ka, and PI3Kc domains [ 47 ], which were present in the deduced MroVps34. Vps34/PI3KC3 is essays physics known to interact with other autophagic proteins such as Atg14 and UV radiation resistance-associated gene (UVRAG) [ 48 , 49 ]. Essay. Since Atg14 and UVRAG proteins were found in M. rosenbergii transcriptomes (Table 1 ) and the predicted structural conformation of M. Essays Physics. rosenbergii Vps34 is similar to human Vps34 (Fig. My Family In Spanish. 2b ), we suggest that MroVps34 may exhibit a similar catalytic activity and bind to Atg14 and essays, UVRAG. Atg8/MAP1LC3B is a protein that is inserted into the nascent autophagosomal membranes upon post-translational lipidation of the reaction writing tips, microtubule-associated precursor [ 50 ]. Both length and amino acid composition were found to essays physics, be similar among representative Atg8/MAP1LC3 sequences from essay in spanish, different animal species (Fig. Essays Physics. 3 ). This suggested that this protein is highly conserved among crustaceans and, moreover, among various animals from different phyla. Consistent with this, the predicted structural conformation of M. rosenbergii Atg8 exhibits a similar secondary structure to that of human MAP1LC3B, including within the regions that are known to be important for binding with Atg7 and personal essay about, tubulin proteins [ 51 , 52 , 53 , 54 ]. Essays Physics. Besides, Atg8/MAP1LC3B normally interacts with other crucial autophagic receptors, for essay about my family in spanish, instance, Atg19, p62, and Atg32, by using two specific hydrophobic pockets, called W- and L-sites (since these two pockets interact with conserved tryptophan and leucine residues within the LIR domain [ 32 ]). We have shown that the amino acid residues known to be important for MAP1LC3B interaction with other proteins are preserved in crustacean Atg8, suggesting that they are critical for Atg8/MAP1LC3B functioning not only in vertebrates but also in crustaceans. p62/SQSTM1 is essays a ubiquitin-binding protein that bridges the autophagy substrate to LC3 through its LIR domain [ 55 ]. Three distinct domains significant for p62 activity include a PB1 domain located at the N-terminus that is responsible for environment, p62 self-assembly [ 56 , 57 ]; a UBA domain located at the C-terminus that is important for essays physics, p62-ubiquitin interacting [ 33 , 58 ]; and a LIR domain that provides an buy answers, interaction site for binding with Atg8/MAP1LC3B [ 55 ]. We found that p62 in crustaceans are not well conserved with other vertebrate p62, both in physics, their length and amino acid composition. A large amino acid insertion could be observed within the middle region of the crustacean p62 when compared to vertebrate p62 sequences. In regard to the LIR motif, this region is mba admission essay buy answers highly conserved among vertebrate species but less conservation could be observed in invertebrate p62 proteins (Fig.

4b ). However, we speculate that the important amino acids ‘WXXL’ (where X is essays physics any amino acid) within LIR, which binds directly to about bullying, MAP1LC3B in physics, mammals [ 32 ], are still preserved in mba admission, invertebrate p62, including M. rosenbergii , with an exception for the leucine residue which is physics substituted by other aliphatic amino acids such as valine or isoleucine. Therefore, it is possible that invertebrate p62 may still have binding activity to personal about, MAP1LC3B similar to vertebrate p62. Lamp-1 is an integral membrane protein of the endosomes and essays, lysosomes, which is involved in the fusion of these organelles with autophagosomal vacuoles [ 31 ]. Despite low similarity in environment, amino acid composition, we found that the MroLamp-1 preserves basic characteristics of Lamp-1 [ 59 ], including a conserved luminal Lamp domain (namely, the physics, C-terminal TM-EGFR domain that extends to reaction, a short cytoplasmic tail). While other vertebrate Lamp-1 contains eight cysteine residues within the physics, luminal region, hence forming four disulfide bonds [ 59 ], MroLamp-1 luminal domain contains only four cysteine residues potentially forming two disulfide bonds, and therefore likely not showing the looped-luminal domain with free-moving hinge as present in mba admission essay, vertebrate Lamp-1 [ 59 ]. Considering a cytoplasmic tail of Lamp-1, this region contains the tyrosine-based motif (Y-X-X-I, where X is a polar residue) important for targeting Lamp-1 to lysosomes [ 60 , 61 , 62 ]. We found that all predicted crustacean Lamp-1 display that significant motif, though their last residues were substituted by essays valine. Essay Newspaper. Although the intracellular targeting of Lamp-1 was conserved among crustaceans and essays physics, other animals, a variable amino acid sequence within the N-terminal part and distinct Lamp domain characteristics suggests a different functional activity of crustacean Lamp-1 from those of vertebrates. Based on sequence alignment, we found that the sequences of M. Paper Tips. rosenbergii and essays physics, human proteins at the regions used for producing anti-BECN1, anti-LC3, anti-p62 and anti-Lamp-1 antibodies shared 58.82, 42.86, 41.18 and 23.37 % identity, respectively, suggesting that antibodies against the human autophagy proteins could be used to detect autophagic proteins in M. rosenbergii . We therefore employed immunoblotting and immunocytochemistry techniques to validate the expression and subcellular localization of Atg marker proteins in nyu admission, M. rosenbergii tissues. Autophagy has been shown to be present in essays, the epithelial cells of the hepatopancreas and/or intestine in personal about bullying, different crustacean species, including Astacus astacus , Eubranchipus grubii and Neocaridina heteropoda , in which autophagic activity was suggested to be essential for digestive cell survival (by eliminating degenerated organelles such as mitochondria) and a proper function of the essays physics, digestive organs [ 16 , 18 , 19 ]. In our study, we found expression of Atg6/BECN1, Atg8/LC3, p62/SQSTM1 proteins to be widespread in mba admission essay, various tissues of essays M. rosenbergii that were investigated (Fig. 6 ). Personal Narrative Essay Bullying. Regarding Atg8/LC3 expression, there were three immunoreactive bands detected in prawn tissues. The largest (18 kDa) and smallest (16 kDa) bands may be considered as the LC3-I and-II forms, as compared with the essays physics, LC3 expression in the human cell sample (Fig.

6 ). The middle band, which is personal narrative about present at an apparent molecular weight of essays physics 17.5 kDa in some tissue samples, probably represents the persuasive essay, intermediate LC3-I that precedes the lapidated form of 16 kDa LC3-II [ 63 ]. Therefore, we suggest that the essays, autophagic process is newspaper general to various prawn tissues, although the essays, specific function of this process within different tissues still requires further investigation. In the personal essay about bullying, ovary, we could observe that the levels of Atg6/BECN1, Atg8/LC3, p62/SQSTM1 and physics, Lamp-1 expression within the cytoplasm increased in correlation with oocyte maturation, while no immunoreactivity was found in the follicular cells. Mba Admission Buy Answers. We hypothesize that the autophagy process may be essential for essays physics, reproductive activity in crustaceans. However, whether autophagy is a part of cellular controls that regulate oocyte differentiation requires further investigation. The present data supports the high evolutionary conservation of reaction writing tips autophagic components in the crustacean lineage, as implicated by essays the existence of several key autophagy-related genes and proteins, and a high degree of sequence conservation of crucial autophagy marker proteins among crustaceans and other representative species from different phyla. This reflects a selection pressure for autophagic process during crustacean evolution. In addition, we have demonstrated that antibodies raised against human autophagy marker proteins can be used for mba admission essay buy answers, monitoring the autophagic process in a crustacean model. Learning how autophagy is essays regulated in the giant freshwater prawn could open up new avenues for novel management in limiting infections, organ stress, as well as nutritional and metabolic imbalances due to feeding and starvation, which can ultimately impact on personal narrative about crustacean aquaculture. Adult females M. rosenbergii were obtained from a local commercial farm in essays, Ayutthaya province, Thailand.

The prawns were anesthetized by immersion in ice water, and personal about bullying, then the ovaries, hepatopancreases, muscles, brains, eyestalks, and physics, thoracic ganglia were dissected out. Fresh tissues were kept at ?80 °C for nyu admission, further use in transcriptome production and Western blotting (described below). For histological and immunohistochemical studies, tissues were fixed in ice-cold 4 % paraformaldehyde for 24 h. After washing with 70 % ethanol five times, specimens were then dehydrated in increasing concentrations of ethanol, cleared in essays, xylene and infiltrated with paraffin, using an automated tissue processor. The paraffin-embedded ovarian tissue blocks were cut at 5 ?m thickness and prepared for immunofluorescence staining. Transcriptome production and personal narrative about, de novo assemblies of CNS and physics, ovary of M. rosenbergii. Transcriptome data of M. rosenbergii was obtained from a previous study [ 26 ]. Reaction Paper Tips. For transcriptome production, in brief, total RNA was isolated from the CNS (inclusive of the eyestalk, brain, thoracic ganglia, and abdominal ganglia) and the ovarian tissues using Tripure isolation reagent (Roche, IN, USA). The quality of total RNA of each tissue was checked by gel electrophoresis and essays, spectrophotometry (NanoDrop 1000; Thermo Fisher Scientific, DE, USA). Tips. Twenty micrograms of total RNA of each tissue was sent to physics, BGI, Hong Kong, for library construction. Briefly, complementary DNA (cDNA) was synthesized from purified and fragmented RNA samples using BGI standard workflow for de novo RNA-seq transcriptomes ( http://bgiamericas.com/ ). The cDNA libraries were constructed based on polymerase chain reaction (PCR) amplification using random hexamer-primed cDNAs. Then the samples were sequenced using Illumina HiSeq 2000 instrument (Illumina Inc.) and only clean reads were used for further sequence assembly. De novo assembly was generated by SOAPdenovo software [ 64 ] using a combined CNS and ovary dataset with parameter set as follows: seqType, fq; minimum kmer coverage = 4; minimum contig length of 100 bp; group pair distance = 250.

All sequence data of M. rosenbergii CNS and ovary transcriptomes were deposited in the NCBI database (Accession number: SRP049917). Gene mining, characterization, and sequence alignment. Searching of Atg proteins in M. rosenbergii transcriptome dataset was carried out by using tBLASTn program in CLC Main Workbench Version 6.0 (CLC Bio-Qaigen, AsiaPac, Taiwan) for which known Atg proteins (from mammals, yeast, and insects) were used as queries. The parameter was set as follows: matrix, BLOSUM62; e-value, 100. Reaction Paper Writing Tips. To search for transcripts encoding Atg proteins in other crustaceans, we used protein queries of known Atg proteins to search within publicly accessible databases [non-redundant (Nr), expressed sequence tags (ESTs), transcriptome shotgun assembly, and Daphnia genome [ 65 ] databases] by using NCBI tBLASTn program ( http://blast.ncbi.nlm.nih.gov/Blast.cgi ), which was restricted to crustaceans (taxid: 6657) and the parameter was set as follows: expect threshold = 10; matrix, BLOSUM62; gap costs, existence = 11 and extension = 1. Nucleotide sequences obtained from BLAST searches were translated into amino acid sequences using the essays physics, CLC Main Workbench Version 6.0 and paper tips, then primary sequence analyzed manually based on homology to other known proteins. Protein alignment of crustacean Atg proteins with other previously known Atg proteins was performed using MEGA6 [ 66 ] and physics, GeneDoc [ 67 ] softwares. Amino acid sequences used in the alignments, as well as their accession numbers, are provided in Additional file 2 . All deduced M. rosenbergii Atg proteins were finally used for BLASTP search against the Nr protein database for further sequence confirmation. The BLAST hit with lowest e-value for each M. rosenbergii Atg protein is buy answers detailed in Additional file 1 . Prediction of essays protein tertiary structure and critical sites. The tertiary structure of selected Atg proteins were predicted using the online tool Protein Homology/analogY Recognition Engine V 2.0 [ 68 ]. Visualization of predicted protein tertiary structure as a 3 dimensional model, illustration of hydrophobicity, superimposition of essay proteins, and structure labeling were performed using the UCSF Chimera version 1.8.1 [ 69 ]. The specific domains and critical sites were predicted by InterPro [ 70 ]. Primary antibodies used in western blotting and immunohistochemistry.

Rabbit polyclonal antibody against BECN1 (Sigma-Aldrich, USA) was produced using synthetic peptide corresponding to amino acid positions 329–345 of human BECN1 as immunogen. Rabbit polyclonal antibody against essays physics LC3B (Sigma-Aldrich, USA) was raised against the human LC3B at amino acid positions 2–15. Rabbit polyclonal antibody against p62 (Santa Cruz, Biotechnology, USA) was produced against the human SQSTM1/p62 at amino acid positions 375–425. Mouse monoclonal antibody against Lamp-1 (BD Science) was produced against human Lamp-1 at about, the amino acid positions 25–224. Western blot analysis of Atg8/LC3, Atg6/BECN1, and physics, p62/SQSTM1. Fresh tissues were homogenized and sonicated in a lysis buffer (0.2 % NaDoc, 1 mM Na 3 VO 4 and 50 mM NaF) and then centrifuged at 10,000? g , 4 °C, for 1 h to nyu admission prompts, obtain tissue extracts.

The supernatants were collected and protein concentrations measured with the essays, Bradford reagent (Sigma). For a positive control, human squamous carcinoma cells (A431 cell line) were sonicated in personal narrative about, lysis buffer and protein concentrations measured. Subsequently, 30 ?g of essays physics total proteins were separated on essay buy answers a 15 % SDS-PAGE gel and transferred onto a polyvinylidene fluoride membrane (GE Healthcare Life Sciences). Nonspecific binding sites were blocked with a blocking buffer [5 % non-fat dry milk in 0.2 % Tween 20 in 0.1 M phosphate-buffered saline (PBS), pH 7.4] for 2 h at room temperature. Physics. The membranes were incubated overnight at 4 °C with the tips, following primary antibodies: either rabbit anti-LC3 (dilution 1:500), rabbit anti-p62/SQSTM1 (dilution 1:200), or rabbit anti-BECN1 (dilution 1:500). The membranes were washed three times for essays, 5 min each with washing buffer (0.01 % Tween 20 in 0.1 M PBS) and about my family, then incubated for 2 h at room temperature in essays, the peroxidase-conjugated secondary antibody [goat anti-rabbit IgG-HRP (SouthernBiotech) dilution 1:5000]. Antigen-antibody binding was detected using an enhanced chemiluminescent substrate (Thermo Scientific) and reaction writing, exposed to Amersham Hyperfilm ECL (GE Healthcare Life Sciences). Immunoperoxidase staining of Atg proteins in the ovary of M. rosenbergii. Immunoperoxidase staining was carried out following a previously described protocol [ 71 ] with some modifications.

Briefly, ovarian sections were sequentially deparaffinized and rehydrated in xylene and decreasing concentrations of ethanol. Endogenous peroxidase was blocked by immersing sections in 3 % H 2 O 2 in methanol for 30 min. Subsequently, the sections were immersed in 1 % glycine in 0.1 M PBS to block free aldehyde groups and then incubated in a blocking serum (10 % bovine serum albumin in physics, 0.1 M PBS, pH 7.4) for 2 h at 4 °C to block non-specific bindings. Essay About My Family. Sections were incubated overnight at essays physics, 4 °C with either rabbit anti-LC3 (dilution 1:500), mouse monoclonal anti-Lamp-1 (dilution 1:500), rabbit polyclonal anti-p62/SQSTM1 (dilution 1:500), or rabbit polyclonal antibody anti-BECN1 (dilution 1:500), in blocking serum. Paper. After washing with PBS, sections were incubated in secondary antibody, either goat anti-rabbit IgG-HRP (Abcam) or goat anti-mouse IgG-HRP (SouthenBiotech) at the dilution 1:500 in blocking serum, for 2 h at room temperature. Negative controls were performed by omitting the primary antibodies. The color was developed with an essays physics, AEC substrate kit (Invitrogen). Finally, sections were observed and photographed under the Nikon E600 light microscope. BECN1, Beclin 1; Lamp-1, lysosomal-associated membrane protein 1Vps, vacuolar protein sorting; MAP1LC3, microtubule-associated proteins 1A/1B light chain 3; SQSTM1, sequestosome. This research was supported by narrative essay a grant from the Office of Higher Education Commission (OHEC) and Mahidol University (MU) under the National Research University Initiatives, an OHEC-MU and the Thailand Research Fund (TRF) Distinguished Research Professor Grant to Professor Prasert Sobhon, a TRF-RGJ Ph.D. Scholarships to Saowaros Suwansa-ard and Wilairat Kankuan; and an ARC grant to Dr.

Scott Cummins. We also thank the financial support from the Consorzio InterUniversitario per le Biotecnologie (Trieste, Italy) and from the essays, Associazione per la Ricerca Medica-Ippocrate-Rhazi (Novara, Italy) to Miss Wilairat Kankuan. This work was supported by a grant from the Office of Higher Education Commission (OHEC) and Mahidol University (MU) under the National Research University Initiatives, an OHEC-MU and the Thailand Research Fund (TRF) Distinguished Research Professor Grant to PS. SS was supported by a TRF-RGJ Ph.D. Tips. Scholarships. WK was supported by a TRF-RGJ Ph.D. Scholarship, the Consorzio InterUniversitario per essays physics le Biotecnologie (Trieste, Italy), and the Associazione per la Ricerca Medica-Ippocrate-Rhazi (Novara, Italy). SFC was supported by an ARC grant. Availability of data and materials. The datasets supporting the conclusions of personal narrative essay about this article are available in the NCBI Sequence Read Archive (SRA) depository (SRA Accession number: SRP049917; http://trace.ncbi.nlm.nih.gov/Traces/sra/?study=SRP049917 ). Physics. Additional supporting data are included as additional files. SS performed the bioinformatics analyses and wrote the manuscript.

WK performed the personal narrative, Western blot analysis and immunohistochemistry, and wrote the manuscript. TT, JS, NK, TK and KK contributed the transcriptomic data and analysis. SFC, CI, and PS provided conception, laboratory support, critical revision of the manuscript, and final approval of the essays, version to be published. The authors have declared that no competing interests exist. Ethics approval and consent to participate. Ethics is not required for prompts, the study. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and essays physics, indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/ ) applies to the data made available in this article, unless otherwise stated. Saowaros Suwansa-ard 1 4 Wilairat Kankuan 1 5 Tipsuda Thongbuakaew 1 7 Jirawat Saetan 2 Napamanee Kornthong 3 Thanapong Kruangkum 1 Kanjana Khornchatri 1 Scott F. Mba Admission. Cummins 4 Ciro Isidoro 5 Email author Prasert Sobhon 1 6 Email authorView author's OrcID profile 1. Physics. Department of essay Anatomy, Faculty of Science Mahidol University Bangkok Thailand 2. Department of physics Anatomy, Faculty of Science Prince of in spanish Songkla University Hatyai Thailand 3. Essays. Chulabhorn International College of narrative about bullying Medicine Thammsat University Phathumthani Thailand 4. Faculty of Science, Health, Education and Engineering University of the Sunshine Coast Maroochydore Australia 5. Laboratory of Molecular Pathology, Department of Health Sciences Amedeo Avogadro University Novara Italy 6. Faculty of Allied Health Sciences Burapha University Chonburi Thailand 7. School of Medicine Walailak University Nakhon Si Thammarat Thailand. .RIS Papers Reference Manager RefWorks Zotero. .BIB BibTeX JabRef Mendeley.

.RIS Papers Reference Manager RefWorks Zotero. .BIB BibTeX JabRef Mendeley. Over 10 million scientific documents at your fingertips. 2017 Springer International Publishing AG. Essays. Part of Springer Nature.

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